Blasticidin S HCl is a peptidyl nucleoside produced by several species ofStreptomycesthat was first isolated fromS. griseochromogenesin 1958. Blasticidin S inhibits protein synthesis and is active against bacteria, fungi, nematodes, and tumor cells. The compound is used as aselection antibiotic for both eukaryotic and prokaryotic cells, and a markerfor strain manipulation.

TOKU-E carries three forms of Blasticidin S:

• Blasticidin S HCl (B001)
• Blasticidin S (B052)
• Blasticidin S HCl Solution (10 mg/ml in 20 mM HEPES)(B006-B007)

Blasticidin S is soluble in water (5-10 mg/ml) and acetic acid.

This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees.

CAS Number

3513-03-9

Molecular Formula

C₁₇H₂₆N₈O₅ · HCl

Molecular Weight

458.90

Mechanism of Action

Blasticidin S HCl inhibits protein synthesis in prokaryotic and eukaryotic cells by binding to the ribosomal P-site which strengthens tRNA binding and slows down and prevents subsequent peptide synthesis.

Mechanisms of resistance

Resistance to blasticidin S is conferred by bsr, BSD, and bls resistance genes isolated from Bacillus cereus K55-S1, Aspergillus terreus, and Streptoverticillum spp, respectively.

The bsr resistance gene is a 420 bp fragment and encodes a 15 kDa blasticidin S deaminase which catalyzes the reaction of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes.

The bsd resistance gene is a 393 bp fragment and also encodes a blasticidin S deaminase enzyme which catalyzes a similar reaction to the BSR deaminase. A study by Kimura et al. found the transfection frequency with bsd to be 80X greater than with bsr when using FM3A cells.

The bls gene resistance gene encodes an acetyltransferase which interacts with acetyl-coenzyme A and prevents blasticidin S from inhibiting protein synthesis.

Storage Conditions

2-8°C;

Tariff Code

2934.99.9000

Spectrum

Blasticidin S HCl is biologically active against susceptible mammalian and prokaryotic cells.

TOKU-E公司1981年成立于日本东京，由来自全球的顶尖专业人士组成，拥有世界领先的体外抗生素生产技术，并快速成长为世界领先的超纯抗生素供应者，以低廉的价格提供最全面的超纯抗生素，其纯度大于99%，用于动物细胞培养、植物组织培养、微生物、抗真菌及诊断等科研领域，允许研究者提高抗生素浓度，并消除污染，降低对细胞的伤害，从而提高筛选效率；该公司还提供精细化学试剂，如超纯丙烯酰胺、无尘SDS、胍盐等，极大提高了实验的精确性和安全性，可满足生命科学研究领域各科研机构，标准化组织机构的高标准需求。