Hygromycin B is a unique aminoglycoside antibiotic derived fromStreptomyces hygroscopicus. Hygromycin B is routinely used as a selection agent in transfection experiments.

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CAS Number

31282-04-9

Molecular Formula

C₂₀H₃₇N₃O₁₃

Molecular Weight

527.52

Mechanism of Action

Hygromycin B, along with aminoglycosides, inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism.

Mechanism of resistance:

Hygromycin B resistance is conferred by the hph gene isolated from Streptomyces hygroscopicus , a 1467 bp fragment which encodes hygromycin B phosphotransferase (HPh). Cell lines successfully transfected with the hph gene produce hygromycin B phosphotransferase and convert hygromycin B to 7”-O-phosphoryl-hygromycin B by phosphorylating the 4-hydroxyl group on the cyclitol ring of hygromycin B. 7”-O-phosphoryl-hygromycin B lacks antibiotic activity and does not interact with prokaryotic or eukaryotic ribosomes.

Storage Conditions

2-8°C

Tariff Code

2941.90.1010

Spectrum

Hygromycin B is effective against eukaryotic (mammalian) and prokaryotic (bacteria, fungi/yeast) cells.

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